Chromatography

Chromatography Quiz Crafted by- 

Akshita Verma 

Lecturer

Department of Forensic Science

Jain (deemed-to-be) University, Bangalore


Let’s have a look on answers of the quiz questions.


Que 1. Chromatography, the “the color writing” was first employed by which Russian Scientist in 1900?

a) Mikhail Tsvet

b) Archer John Porter Martin

c) Richard Willstatter

d) Richard Laurence Millington Synge

Answer: a) Mikhail Tsvet


Que 2. Characteristic feature of any form of chromatography is?

a) Use of molecules that are soluble in water

b) Use of an inert carrier gas

c) Use of a mobile and a stationary phase

d) Calculation of an Rf value for the molecules separate

Answer: c) Use of a mobile and a stationary phase


Que 3. Which of the following is not the principle of Chromatography?

a) Adsorption

b) Absorption

c) Affinity

d) Partition

Answer: b) Absorption


Que 4. Removal of water or other solvents through the TLC plate is called as?

a) Elution

b) Adsorption

c) Diffusion

d) Activation

Answer: d) Activation


Que 5. The sensitivity of TLC is of the order?

a) Milligram

b) Microgram

c) Nanogram

d) Picogram

Answer: b) Microgram


Que 6. In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

a) Column chromatography

b) Planar chromatography

c) Liquid chromatography

d) Gas chromatography

Answer: a) Column chromatography


Que 7. Paper Chromatography generally uses?

a) One phase solvent system

b) Two phase solvent system

c) Three phase solvent system

d)  All of the above

Answer: a) One phase solvent system


Que 8. Which of the following cannot be used as adsorbent in Column adsorption chromatography?

a) Magnesium oxide

b) Silica gel

c) Activated alumina

d) Potassium permanganate

Answer: d) Potassium permanganate


Que 9. If the mobile phase is gas, movement of solute is determined by its?

a) Melting point

b) Boiling point

c) Volatility

d) Solubility

Answer: c) Volatility


Que 10. In reverse phase chromatography, the stationary phase is made?

a) Polar

b) Non polar

c) Both

d) None

Answer: b) Non polar


Que 11. The process of passing a mobile phase through a chromatography column is called as?

a) Flushing

b) Washing

c) Elution

d) Partitioning

Answer: c) Elution


Que 12. Most modern sample injection system in HPLC is?

a) Microvolume sampling valve

b) Syringe injection

c) Stop flow injection

d) All of the above

Answer: a) Microvolume sampling valve


Que 13. Which chromatography is using a salt gradient or a pH gradient?

a) Ion exchange chromatography

b) High Performance Liquid Chromatography

c) Thin Layer Chromatography

d) Gas Chromatography

Answer: a) Ion exchange chromatography


Que 14. Thermostat in HPLC regulate the temperature of

a) Solvent Reservoir

b) Column

c) Detector

d) Sample inlet

Answer: b) Column


Que 15. The gradient elution in HPLC is one in which the component of solvent?

a) Remains constant

b) Changes continuously or in series of step

c) Both of the above

d) None of the above

Answer: b) Changes continuously or in series of step

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