RNA Profiling and Challenges

RNA Profiling and Challenges Quiz Crafted By-

Govind

Scientific Officer

Sherlock Institute of Forensic Science India Pvt. Ltd, New Delhi

Introduction to RNA Profiling and Challenges

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.

Let's look at the questions and their answer. 

Ques 1. How is the nucleotide sequence in the anti-codon arm of t-RNA?

a) Same as in codon

b) Opposite to the codon sequence

c) Not constant

d) None of the above

Answer-b) Opposite to the codon sequence

Ques 2. Which of the following is the function of r-RNA?

a) Maintains shape of ribosome                                                      

b) Protein synthesis

c) Binds m-RNA and ribosome                                                        

d) All of the above

Answer- d) All of the above

Ques 3. Which of the following statement is NOT true?

a) In prokaryotes, mostly the m-RNA is mono-cistronic                                                      

b) The acceptor arm consist of base paired stem

c) r-RNA perform some of the enzymatic activity                                                        

d) Prokaryotic ribosome contains 3 r-RNA

Answer- a) In prokaryotes, mostly the m-RNA is mono-cistronic

Ques 4. Which of the following serves as template for protein synthesis?

a) t-RNA                                                      

b) r-RNA

c) m-RNA itself                                                        

d) None of the above

Answer- c) m-RNA

Ques 5. The eukaryotic ribosome contains how many r-RNA molecules?

a) 3                                                      

b) 2

c) 4                                                       

d) 6

Answer- c) 4

Ques 6. Which of the following binds the m-RNA to the ribosome?

a) t-RNA                                                      

b) Nucleus

c) m-RNA itself                                                        

d) None of the above

Answer- d) None of the above

Ques 7. Which of the following are correctly Matched 

a) m-RNA - Carries the protein blueprint from a cell's DNA to its ribosomes                                                      

a) t-RNA - Carries active amino acid to ribosomes 

b) r-RNA - Serves as template for protein synthesis

c) All are correctly Matched                                                        

Answer- d)All are correctly Matched           

Ques 8. The m-RNA is synthesized in the nucleus in which form?

a) Heterogenous RNA                                                      

b) Homogenous RNA

c) Homologous RNA                                                        

d) None of the above

Answer- a) Heterogenous RNA

Ques 9. Which type of sugar is present in RNA?

a) Pentose sugar                                                      

b) Ribose sugar

c) Deoxy-ribose sugar                                                      

d) Both B and C

Answer- b) Ribose sugar 

Ques 10. Which of the following base is not present in RNA?

a) Cytosine                                                      

b) Thymine

c) Uracil                                                        

d) Guanine

Answer- b) Thymine